Journal: Bioengineered

Article Title: Curcumol enhances cisplatin sensitivity of gastric cancer: involvement of microRNA-7 and the nuclear factor-kappa B/snail family transcriptional repressor 1 axis

doi: 10.1080/21655979.2022.2070975

Figure Lengend Snippet: Curcumol upregulates miR-7 expression in GC cells. a, miRNAs with differential expression in cells after curcumol treatment identified by microarray analysis; b, miR-7 expression in cells after 80 μM curcumol treatment examined by RT-qPCR (* p < 0.05; two-way ANOVA); c, miR-7 expression in tumor and the paired normal tissues evaluated by RT-qPCR (n = 33; * p < 0.05; the unpaired t test); d, correlation between miR-7 expression and the patient’s survival (* p < 0.05; Kaplan-Meier analysis); e, miR-7 expression in MKN45 and HGC27 cells after different doses of curcumol treatment examined by RT-qPCR (* p < 0.05, ** p < 0.01; two-way ANOVA).

Article Snippet: This reaction was performed at 37°C for 30 min. After that, the labeled RNA was hybridized with Human miRNA Expression Microarray V4.0 (Arraystar, Rockville, MD, USA) for 24 h. The gene expression data were obtained using a GeneChip TM Scanner 3000 7 G system (#00-0210, 2008, Thermo Fisher Scientific) and analyzed by the R Language Program (Version 3.6.3, R).

Techniques: Expressing, Quantitative Proteomics, Microarray, Quantitative RT-PCR

Journal: American Journal of Translational Research

Article Title: The pseudogene URAHP promotes proliferation and regulates the pathogenesis of preeclampsia

doi:

Figure Lengend Snippet: Elevated expression of lncRNA URAHP in preeclampsia placenta tissues. (A and B) Differentially expressed lncRNAs or mRNA in 3 pairs of placental tissues from women with PE and normal pregnancy (Con). (C) The Transcriptional expression of lncRNA URAHP in placental tissues from women with PE and normal pregnancy (Con) examined by real-time PCR, and (D) indicates lncRNA URAHP in HTR-8/SVneo, JAR and JEG-3 cell lines. All the experiments were carried out for three times. *P < 0.05; **P < 0.01. β-actin was used as a loading control.

Article Snippet: The sample preparation and microarray hybridization were performed according to the manufacturer’s standard protocols with minor modifications (Arraystar Human LncRNA V4.0 analysis) [ 17 ].

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Journal: Functional & Integrative Genomics

Article Title: Comprehensive analysis of microarray expression profiles of circRNAs and lncRNAs with associated co-expression networks in human colorectal cancer

doi: 10.1007/s10142-018-0641-9

Figure Lengend Snippet: Differentially expressed circular RNA (circRNAs) in human CRC tissues (A) and adjacent normal tissues (N). a Hierarchical clustering of differentially expressed circRNAs. b The box plot shows the variations in circRNA expression. c The scatter plot explains the distribution of the data in circRNA profiles. The values of the x - and y -axes in the scatter plot were the averaged normalized signal values of the group (log 2 scales). d The volcano plot illustrates the distribution of the data in circRNA profiles. The green lines in the scatter and volcano plots show the significant fold change (color figure online)

Article Snippet: Briefly, mRNA was purified from total RNA, amplified, transcribed into fluorescent cRNA, and then hybridized into the Human circRNA microarray V2 (Arraystar) and Human lncRNA microarray V4.0 (Arraystar).

Techniques: Expressing

Journal: Functional & Integrative Genomics

Article Title: Comprehensive analysis of microarray expression profiles of circRNAs and lncRNAs with associated co-expression networks in human colorectal cancer

doi: 10.1007/s10142-018-0641-9

Figure Lengend Snippet: Classification of differentially expressed circRNAs and lncRNAs in CRC tissues. a Types and counts of differentially regulated circRNAs detected by microarray (fold change ≥ 2.0, P < 0.05 and FDR < 0.05). The circRNAs are classified into five types according to the relationship and genomic loci with their associated coding genes. b Types and counts of differentially regulated lncRNAs detected by microarray (fold change ≥ 2.0, P < 0.05 and FDR < 0.05). The lncRNAs are classified into six types according to the relationship and genomic loci with their associated coding genes

Article Snippet: Briefly, mRNA was purified from total RNA, amplified, transcribed into fluorescent cRNA, and then hybridized into the Human circRNA microarray V2 (Arraystar) and Human lncRNA microarray V4.0 (Arraystar).

Techniques: Microarray

Journal: Functional & Integrative Genomics

Article Title: Comprehensive analysis of microarray expression profiles of circRNAs and lncRNAs with associated co-expression networks in human colorectal cancer

doi: 10.1007/s10142-018-0641-9

Figure Lengend Snippet: Differentially expressed long non-coding RNAs (lncRNAs) in human CRC tissues (A) and adjacent normal tissues (N). a Hierarchical clustering of differentially expressed lncRNAs. b The box plot shows the variations in circRNA expression. c The scatter plot explains the distribution of the data in lncRNA profiles. The values of the x - and y -axes in the scatter plot were the averaged normalized signal values of the group (log 2 scales). d The volcano plot illustrates the distribution of the data in lncRNA profiles. Red, green, and black points in the scatter and volcano plots represent significant upregulated lncRNAs; significant downregulated lncRNAs and not differential expressed lncRNAs, respectively (color figure online)

Article Snippet: Briefly, mRNA was purified from total RNA, amplified, transcribed into fluorescent cRNA, and then hybridized into the Human circRNA microarray V2 (Arraystar) and Human lncRNA microarray V4.0 (Arraystar).

Techniques: Expressing

Journal: Functional & Integrative Genomics

Article Title: Comprehensive analysis of microarray expression profiles of circRNAs and lncRNAs with associated co-expression networks in human colorectal cancer

doi: 10.1007/s10142-018-0641-9

Figure Lengend Snippet: Differentially expressed mRNAs in human CRC tissues (A) and adjacent normal tissues (N). a Hierarchical clustering of differentially expressed mRNAs. b The box plot shows the variations in circRNA expression. c The scatter plot explains the distribution of the data in mRNA profiles. The values of the x - and y -axes in the scatter plot were the averaged normalized signal values of the group (log 2 scales). d The volcano plot illustrate the distribution of the data in mRNA profiles. Red, green and black points in the scatter and volcano plots represent significant upregulated mRNAs, significant downregulated mRNAs and not differential expressed mRNAs, respectively (color figure online)

Article Snippet: Briefly, mRNA was purified from total RNA, amplified, transcribed into fluorescent cRNA, and then hybridized into the Human circRNA microarray V2 (Arraystar) and Human lncRNA microarray V4.0 (Arraystar).

Techniques: Expressing

Journal: Functional & Integrative Genomics

Article Title: Comprehensive analysis of microarray expression profiles of circRNAs and lncRNAs with associated co-expression networks in human colorectal cancer

doi: 10.1007/s10142-018-0641-9

Figure Lengend Snippet: Verification for the expression of significant transcripts by qRT-PCR. a–e Relative expression levels of one circRNA, one lncRNA, and three mRNAs are shown comparing tumor tissues (A) and adjacent normal tissues (N). f A good correlation of qRT-PCR results and microarray data are shown through comparing such two results. The heights of the columns stand for the fold changes (log2 transformed) computed from the qRT-PCR and microarray data

Article Snippet: Briefly, mRNA was purified from total RNA, amplified, transcribed into fluorescent cRNA, and then hybridized into the Human circRNA microarray V2 (Arraystar) and Human lncRNA microarray V4.0 (Arraystar).

Techniques: Expressing, Quantitative RT-PCR, Microarray, Transformation Assay

Journal: Functional & Integrative Genomics

Article Title: Comprehensive analysis of microarray expression profiles of circRNAs and lncRNAs with associated co-expression networks in human colorectal cancer

doi: 10.1007/s10142-018-0641-9

Figure Lengend Snippet: Clinical characteristic of 24 CRC patients and the expression of five genes in tissues

Article Snippet: Briefly, mRNA was purified from total RNA, amplified, transcribed into fluorescent cRNA, and then hybridized into the Human circRNA microarray V2 (Arraystar) and Human lncRNA microarray V4.0 (Arraystar).

Techniques: Expressing